Automating Image Analysis
Taking image analysis to a whole new level.
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New Interface, New Features,
Same Analysis You Trust
Pipsqueak Pro is the next evolution of image analysis. Take out the guess work and simplify your detection and quantification workflows with simple yet powerful software.
Using our patented artificial intelligence platform.
SINGLE & MULTI-CHANNEL
Pipsqueak offers both single and multi-label analysis with colocalization….
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RESPONSIVE INTERFACE
Pipsqueak’s interface harnesses the power of our AI engine to delivery the accuracy you expect in a smooth and responsive platform.
DATA EXPORT
Easily export your results with common-seperated values (.csv) files to integrate with Excel or statistical packages.
CUSTOM MODEL TRAINING
With the power of Rewire’s machine learning engine, Sightologist, you can train custom AI models to fit any need.
Reliable, Every Single Time.
[The integration of ML into image analysis] has been shown to improve reproducability and speed of microscopy quantification across both inter- and intra- rater reliability tests.
This doesn’t just mean the same work can be done faster, but that more work can be done in the same time.
DOWNLOAD FOR MACDOWNLOAD FOR WINDOWSBetter, Faster, More Accurate
Pipsqueak is published and proven to increase the accuracy and reliability of your data, while reducing tedious and time-consuming manual cell detection.
Reduction in analysis cost
Faster than by hand
Less data variability
Get Started Today
Free Trial
$0
- No Credit Card Required
- Automatic Cell Detection & Quantification
- Multi Stain Colocalization
- Custom Detection Model Training
Pipsqueak Pro
$869
per year
- 1-Seat, 1-Year License
- Unlimited Automatic Detection
- Unlimited Custom Detection Models
- Multi Stain Colocalization
- Cloud Sync & Storage (coming soon)
- Login at Home or Lab
- Use Pipsqueak Pro & Legacy versions
Multi Seat & multi Year licenses
Request a Quote
- Save on Multi Seats and/or Multi Years
- Cloud SHARE Projects with Members
- Share Custom Detection Models
- See Auditable Analyses from Members
Researcher Testimonials
Don’t take our word for it – here’s what some of our users say:
“Critically, [Pipsqueak] generates reliable and sensitive results that can be easily cross-referenced
by multiple investigators for consistency and bias. In contrast to manual image quantification,
Pipsqueak is capable of analyzing an image in mere minutes versus hours.
In my experience, Pipsqueak is
the best tool currently available to investigators working on perineuronal net analysis in the field of neuroscience.”
Yevgeniya
Johns Hopkins University

“Pipsqueak has become an integral part of my dissertation research, greatly reducing the time needed to conduct image analysis and becoming a staple in the lab that many members use every day.”
Kelsea
Northeastern University

“This software is the gold standard in the field for quantifying the density of these structures. Pipsqueak algorithms excel above other techniques by setting a consistent background and selecting only the fluorescent structures that exceed the background and fulfill parameters input by the user.
This allows for more consistent analysis not only between experiments, but users as well.”
Kristin
Medical University of
South Carolina

“Our recent paper (Reinhard et al., 2019, Neurobiology of Learning and Memory) used
[Pipsqueak] extensively to analyze perineuronal net intensity in the auditory cortex,
hippocampus and amygdala in an autism mouse model.
For my trainees to have conducted this
work without the semi-automated analysis afforded by your program would have delayed the
paper by at least a year.
Pipsqueak has allowed for more consistency between experiments and
personnel by setting constant backgrounds and accurately tagging the cells we are specifically
interested in.”
Khaleel
UC Riverside

Download Pipsqueak Pro Today
Used by people like you.
Pipsqueak was first published in 2016 and has since been used by hundreds of researchers to imporve the speed, accuracy, and reproducibility of their image analysis.
Peer-reviewed citations
See the citations here
Images analysed per month
Join the Pipsqueak Community & enjoy the power of AI-image analysis.
Users around the world
Pipsqueak is used on every continent (except Antartica, because Penguins don’t have microscopes).
Finish Your Project Faster, Without Complication.
Introducting the next evolution of image analysis. Quantification of immunostained images doesn’t have to be hard. Simplify your workflow without paying for expensive and overly complicated programs like Aivia or Imaris.
Pipsqueak ProTM takes out the guess work and automates cell detection and intensity analysis using our patented artificial intelligence platform.

Cloud Sync and Share Projects
Pipsqueak ProTM uses our power of artificial intelligence engine to make histology fast and effortless. Analyze hundreds of your images in fractions of the time.
Share and store projects from your cloud.

Faster Cell Detection with Artificial Intelligence
Count and quantify cells, fluorescent probes, and other targets quickly and effortlessly with automated ROI detection.
With Pipsqueak ProTM, analysis becomes the easy part of your experiment. Customizible AI detection makes it easy to automatically detect and quantify your staining.

Responsive design
We’ve completely rebuilt our interface, but still deliver the accuracy you trust and expect from ImageJ/FIJI. In fact, we built ImageJ’s analysis right into the Pipsqueak Pro interface! Now your trusted analysis workflow will be silky smooth and faster than ever before.
Start your trialOnline Preview
Try our web preview of Pipsqueak Prodetection capability.
See how we can automate your microscopy image analysis.
Work smarter, not harder.
Pipsqueak Pro brings the power of machine learning to your image analysis.
Start your Pipsqueak Pro trial today
Pipsqueak Pro is for scientists, by scientists to improve the speed and reproducibility of your microscopy. Try Pipsqueak Pro, no-risk for 1-month!.
GET STARTED TODAY