Pipsqueak Pro

Automating Image Analysis

Taking image analysis to a whole new level.

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New Interface, New Features,
Same Analysis You Trust

Pipsqueak Pro is the next evolution of image analysis. Take out the guess work and simplify your detection and quantification workflows with simple yet powerful software. 

Using our patented artificial intelligence platform.

SINGLE & MULTI-CHANNEL

Pipsqueak offers both single and multi-label analysis with colocalization….
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RESPONSIVE INTERFACE

Pipsqueak’s interface harnesses the power of our AI engine to delivery the accuracy you expect in a smooth and responsive platform.

DATA EXPORT

Easily export your results with common-seperated values (.csv) files to integrate with Excel or statistical packages.

CUSTOM MODEL TRAINING

With the power of Rewire’s machine learning engine, Sightologist, you can train custom AI models to fit any need.

Reliable, Every Single Time.

[The integration of ML into image analysis] has been shown to improve reproducability and speed of microscopy quantification across both inter- and intra- rater reliability tests.

This doesn’t just mean the same work can be done faster, but that more work can be done in the same time.

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Better, Faster, More Accurate

Pipsqueak is published and proven to increase the accuracy and reliability of your data, while reducing tedious and time-consuming manual cell detection. 

Reduction in analysis cost

Faster than by hand

Less data variability

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Free Trial

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  • No Credit Card Required
  • Automatic Cell Detection & Quantification
  • Multi Stain Colocalization
  • Custom Detection Model Training
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Multi Seat & multi Year licenses

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  • Save on Multi Seats and/or Multi Years
  • Cloud SHARE Projects with Members
  • Share Custom Detection Models
  • See Auditable Analyses from Members
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Researcher Testimonials

Don’t take our word for it – here’s what some of our users say:

“Critically, [Pipsqueak] generates reliable and sensitive results that can be easily cross-referenced
by multiple investigators for consistency and bias. In contrast to manual image quantification, Pipsqueak is capable of analyzing an image in mere minutes versus hours.

In my experience, Pipsqueak is the best tool currently available to investigators working on perineuronal net analysis in the field of neuroscience.”

Yevgeniya

Johns Hopkins University

“Pipsqueak has become an integral part of my dissertation research, greatly reducing the time needed to conduct image analysis and becoming a staple in the lab that many members use every day.”

Kelsea

Northeastern University

“This software is the gold standard in the field for quantifying the density of these structures. Pipsqueak algorithms excel above other techniques by setting a consistent background and selecting only the fluorescent structures that exceed the background and fulfill parameters input by the user.

This allows for more consistent analysis not only between experiments, but users as well.”

Kristin

Medical University of
South Carolina

“Our recent paper (Reinhard et al., 2019, Neurobiology of Learning and Memory) used
[Pipsqueak] extensively to analyze perineuronal net intensity in the auditory cortex, hippocampus and amygdala in an autism mouse model.

For my trainees to have conducted this work without the semi-automated analysis afforded by your program would have delayed the paper by at least a year.

Pipsqueak has allowed for more consistency between experiments and personnel by setting constant backgrounds and accurately tagging the cells we are specifically interested in.”

Khaleel

UC Riverside

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Used by people like you.

Pipsqueak was first published in 2016 and has since been used by hundreds of researchers to imporve the speed, accuracy, and reproducibility of their image analysis.

Peer-reviewed citations

See the citations here

Images analysed per month

Join the Pipsqueak Community & enjoy the power of AI-image analysis.

Users around the world

Pipsqueak is used on every continent (except Antartica, because Penguins don’t have microscopes).

Finish Your Project Faster, Without Complication.

Introducting the next evolution of image analysis. Quantification of immunostained images doesn’t have to be hard. Simplify your workflow without paying for expensive and overly complicated programs like Aivia or Imaris.

Pipsqueak ProTM takes out the guess work and automates cell detection and intensity analysis using our patented artificial intelligence platform.  

Cloud Sync and Share Projects

Pipsqueak ProTM uses our power of artificial intelligence engine to make histology fast and effortless. Analyze hundreds of your images in fractions of the time.
Share and store projects from your cloud.

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Faster Cell Detection with Artificial Intelligence 

Count and quantify cells, fluorescent probes, and other targets quickly and effortlessly with automated ROI detection.
With Pipsqueak ProTM, analysis becomes the easy part of your experiment. Customizible AI detection makes it easy to automatically detect and quantify your staining.

Learn more

Responsive design

We’ve completely rebuilt our interface, but still deliver the accuracy you trust and expect from ImageJ/FIJI. In fact, we built ImageJ’s analysis right into the Pipsqueak Pro interface! Now your trusted analysis workflow will be silky smooth and faster than ever before. 

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Online Preview

Try our web preview of Pipsqueak Prodetection capability.

See how we can automate your microscopy image analysis.

Work smarter, not harder. 
Pipsqueak Pro brings the power of machine learning to your image analysis. 

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Start your Pipsqueak Pro trial today

Pipsqueak Pro is for scientists, by scientists to improve the speed and reproducibility of your microscopy. Try Pipsqueak Pro, no-risk for 1-month!.

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